Vaccines | Free Full-Text | Immune Evasion of SARS-CoV-2 Omicron Subvariants XBB.1.5, XBB.1.16 and EG.5.1 in a Cohort of Older Adults after ChAdOx1-S Vaccination and BA.4/5 Bivalent Booster

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/vaccines12020144/s1. Figure S1: Neutralization against Omicron sublineages BA.4/5, XBB.1.5, XBB.1.16, and EG.5.1 in individuals with or without history of infection by SARS-CoV-2. (A,B) NT₅₀ values of 22 and 37 human sera without and with infection history, respectively, against Omicron BA.4/5, XBB.1.5, XBB.1.16 and EG.5.1, collected after (A) 1 month (median 36 days) post 4th dose with parental ChAdOx1-S vaccine, and (B) 3 months (median 92 days) post 5th dose with bivalent mRNA vaccine. Geometric mean titers (GMTs) are noted above the respective groups. Colors designate infection history, data points represent individual subjects, bars represent GMT with 95% confidence intervals (CI) and dotted lines represent the lower limit of detection of NT₅₀ (LOD = 18). Groups were compared by the non-parametric Mann–Whitney test (ns = not significant, * = p ₅₀ values are the geometric mean (GMT) of triplicate CPE-VNT results (one independent experiment). Titers below the LOD (₅₀, 50% neutralization antibody titer; 95% CI, 95% confidence interval; Table S4: p values calculated from Wilcoxon matched-pairs signed-rank test for group comparison of NT₅₀ geometric mean titers (GMTs) at each time-point from Figure 2B–E. Values > 0.05 (not statistically significant) are highlighted in red; Table S5: Geometric mean titers (GMTs) with 95% CI of neutralizing titers against SARS-CoV-2 ancestral strain (D614G) and Omicron sublineages BA.4/5, XBB.1.5, XBB.1.16, and EG.5.1 at 1 and 4 months post 4th dose with ChAdOx1-S (Oxford/AstraZeneca) and 3 months post 5th dose with Comirnaty Bivalent Original/Omicron BA.4/BA.5 (Pfizer-BioNTech). Individual NT₅₀ values is the geometric mean (GMT) of triplicate CPE-VNT results (one independent experiment). Titers below the LOD (₅₀, 50% neutralization antibody titer; 95% CI, 95% confidence interval. p values calculated from the nonparametric Mann–Whitney test (unpaired) for group comparison of individuals without and with infection history by SARS-CoV-2 of NT₅₀ geometric mean titers (GMTs) at each time-point from Figure 3 and Supplemental Figure S1. p values